DNA

Part:BBa_K3059703

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-21)


F sticky end suffix

Suffix BsaI sticky end F; exposed during Golden Gate to reveal sticky end and allow for attachment to prefix sticky end. This part can be attached to any part that is compatible with 3G assembly and is compatible with CIDAR MoClo.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6


[edit]
Categories
Parameters
None